Mass Cytometry

Predefined panels

The SIgN Mass Cytometry platform has established in- house a comprehensive set of complex panels for analysis of human and mouse immune cell subsets in PBMCs and tissues. Each panel typically comprises of around 40 markers and can be customized according to the specific research question. The panels currently available were specifically designed for deep phenotyping of key immune cell subsets in collaboration with SIgN PIs. Panels developed at SIgN include steady-state and functional analysis of activated cells (e.g cytokine, exhaustion markers etc.). We are continuously developing and testing new panels in-house. For the available list of panels and markers, please see below.

BCL-2, CCR2, CCR4, CCR5, CCR6, CCR7, CCR9, CD103, CD127, CD14, CD16, CD161, CD19, CD25, CD27, CD3, CD38, CD4, CD45, CD45RA, CD45RO, CD49a, CD49d, CD56, CD57, CD8, CLA, CX3CR1, CXCR3, CXCR5, FoxP3, gδTCR, HLA-DR, ICOS, Integrin β7, Ki-67, PD-1, Vα7.2, Vδ1, Vδ2
Napolitani, G., et al. Nature Immunology. 2018 19(7): 742-754
CCR10, CCR2, CCR4, CCR5, CCR6, CCR7, CCR9, CD103, CD127, CD14, CD16, CD161, CD19, CD25, CD27, CD3, CD31, CD38, CD4, CD45, CD45RA, CD45RO, CD49a, CD49d, CD56, CD57, CD62L, CD69, CD8, CD95, CLA, CX3CR1, CXCR3, CXCR4, CXCR5, CXCR6, gδTCR, HLA-DR, ICOS, Integrin β7, PD-1
Wong, MT., et al. Immunity. 2016 45(2): 442-456
2B4, CCR10, CCR2, CCR4, CCR5, CCR6, CCR8, CD103, CD137, CD14, CD15s, Cd16, CD161, CD19, CD25, CD3, CD38, CD39, CD4, CD45, CD45RA, CD45RO, CD49a, CD56, CD57, CD69, CD8, CTLA-4, CXCR3, CXCR5, CXCR6, FoxP3, gδTCR, Helios, HLA-DR, Ki-67, OX40, PD-1, TIGIT, TIM-3, Vα7.2
Not published (developed in-house)
CCR2, CCR4, CCR6, CCR7, CCR9, CD103, CD107a, CD14, CD16, CD161, CD19, CD25, CD3, CD38, CD4, CD40L, CD45, CD45RA, CD56, CD57, CD8a, CTLA-4, CX3CR1, CXCR5, gδTCR, GM-CSF, HLA-DR, ICOS, IFN-γ, IL-10, IL-17A, IL-2, IL-22, IL-4, IL-8, Integrin β7, PD-1, TNF-α, Vα7.2, Vδ1, Vδ2
Napolitani, G., et al. Nature Immunology. 2018 19(7): 742-754

BTLA, CADM1, CCR2, CD10, CD116, CD11b, CD11c, CD123, CD13, CD14, CD141, CD15, CD16, CD163, CD19, CD1c, CD2, CD26, CD303, CD327, CD33, CD34, CD38, CD45, CD45RA, CD48, CD5, CD56, CD62L, CD68, CD7, CD80, CD85j, CD86, Clec9A, CX3CR1, CXCR3, FcER1, HLA-DR
See, P., et al. Science. 2017; 356 (6342): eaag3009
B220, BST2, CCR2, CD103, CD115, CD11A, CD11b, CD11c, CD172a, CD19, CD24, CD25, CD3, CD301, CD38, CD4, CD43, CD44, CD45, CD48, CD54, CD62L, CD64, CD8, CD86, CD90, ESAM, F4/80, FcER1, Ly6c, Ly6G, Ly6GC, MerTK, MHCII, PD-L1, PD-L2, Siglec H, TER119
Becher, B., et al. Nature Immunology. 2014; 15(12): 1181-1189

For enquiries regarding these predefined panels, please contact us by completing the form below (all fields required). Please indicate your panel of interest and/or any additional markers of interest.

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