Immunogenomics

Technology & Applications

Unlock the potential of single cell transcriptomics and immune profiling! The SIgN Immunogenomics platform uses 10x Genomics technology in its single cell analysis. The 10x Genomics Chromium instrument generates a nano-emulsion of single cells each co-encapsulated with a barcoded bead, enabling the RNA of up to ~5000 cells to be analysed in a single tube while maintaining single-cell resolution. Applications include:

  • Gene expression profiling of a heterogeneous cell population, such as a tissue or tumour. Traditional RNA sequencing of bulk tissues provides an average profile of multiple cell types, while single cell transcriptomics reveals the properties of the subpopulations.
  • Within one cell type, single cell transcriptomics can reveal distinct sub-populations with different functions, potentially identifying novel biomarkers or gene signatures.
  • Obtain paired T cell receptor (TCR) and B cell receptor (BCR) sequences to study clonal diversity and clonal expansion. TCR/BCR readouts are linked at the single cell level to the gene expression profile.

Representative tSNE plot of 10X Genomics sequencing data



10x Genomics Service Data Analysis

Contact Us

Immunogenomics Team at SIgN


Single-cell Analysis Team at SIgN



For scientific questions related to immunogenomics, please contact:

Shanshan HOWLAND
Shanshan_Howland@immunol.a-star.edu.sg
Head, Immunogenomics

For scientific questions related to single-cell sequencing data analysis, please contact:

Jinmiao CHEN
Chen_Jinmiao@immunol.a-star.edu.sg
Head, Single-Cell Analysis

For general enquiries/ service engagement, please email: SIgN_IMP@immunol.a-star.edu.sg.

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