Luminex is a multiplex immunoassay that allows for the simultaneous measurement of multiple analytes, such as cytokines, chemokines and growth factors. Similar to Sandwich-ELISA, it is based on pairs of detection- and capture-antibodies, of which the latter are attached to fluorescent-labeled magnetic beads. Currently available kits can measure up to 65 different analytes. There are specialized kits for different areas of study such as inflammation, T cell activation, apoptosis and metabolism. In addition to provision of service with of-the-shelf panels, the MAP platform can help you customize the panels and/or develop new panels that best address your research interest.
At SIgN, we have two FLEXMAP 3D® analyzers, which simultaneously read up to 151 different analytes in a single low-volume sample. Depending on the number of samples to be analyzed, the required precision and the available sample volume, you can choose between three formats. The Standard Format offers the highest accuracy and the most costly, since this assay is carried out strictly according to manufacturer's guidelines, it requires an entire kit per plate (~80 sample wells). The Exploratory Format, is more cost effective as we charge on a per-well basis. This format is currently only offered for three pre-configured human panels (41plex 'Chemokine/Cytokine', 48plex 'Human Cytokine Screening panel' and 65plex 'Human Immune Monitoring'). Please note that the accuracy of the Exploratory Format is lower as compared to the Standard Format. The full set of standards is not run for every test but can be requested for an extra charge. The exploratory format is therefore recommended only if you have a few samples or if you would like to conduct a trial to configure your final panel. Lastly, for low-volume samples, we offer the DropArray® format. It employs droplet-based liquid handling systems developed by Curiox BioSystems and allows the analysis of sample volumes as low as 5μl.
1 Luminex kit includes a 96-well plate, of which 16 wells are used for standards, background control, and quality controls. You can typically run up to 80 single samples or 40 samples in duplicate with a single kit.
Most Luminex kits require 25μl of plasma/serum or 25-50μl of cell culture supernatant. If you have samples that are of low volume,i.e. as low as 5ul, do contact us and we will assist you.
If you select the standard format, you would have to purchase an entire kit but you do not need to use the entire kit in a single run. Several kits provide an extra set of standards which allows for additional runs. However, certain kits only contain 1 set of standards and these are usually stable for 1-3 months at -80c, thus the plate has to be used within that period.
There are many vendors that provide specialized kits for many research purposes with hundreds of targets. They range from Inflammatory, Cancer, Diabetes, Cardiovascular, Toxicity, Neuroscience, T cell, B cell, and many others. There are also fully customizable kits where you can choose your targets of interest if they are not included in the ready-made kits. Let us know your analytes of interest under Luminex Service and we will find the most suitable kits for your research. However, do note that not all targets can be combined in a single kit as there is the possibility of cross reactivity.
Serum, plasma, cell culture supernatant, lysate, urine, saliva, tears, CSF and milk. Sources may be from humans, mice, rats, porcine, canine and non-human primates. Samples to be run on the luminex must be of Biosafety Level 2 classifications. Samples that are transfected or infected with HIV, SIV, HPV, HCV, HSV, HTLV-1, HTLV-2, CMV, SARS, Avian Influenza A, Mycobacterium tuberculosis, Mycobacterium bovis, Neisseria meningitidis, Mycoplasma are not permitted. However, exceptions may be made if the samples are appropriately treated to inactivate the infectious agents, and there is full disclosure of the experimental methodology and inactivation protocol. The aforementioned exceptions will be assessed on a case-by-case scenario. In addition, samples that are radioactive or labeled with radioactive reagents are prohibited.