Multiplex Analysis of Proteins (MAP)

Technology & Applications

The SIgN Multiplex Analysis of Proteins (MAP) platform employs the use of multiple technologies to support high-throughput and simultaneous screening of protein targets/analytes from a single sample. The technologies employed by the MAP platform enable screening of potential biomarkers in large clinical cohorts and immunomonitoring research applications. More specifically, the analysis of proteins can be applied in the study of inflammation, T-cell activation, apoptosis and metabolism. The MAP platform has experience in analysing proteins in a variety of human samples including plasma, serum, urine, tears, breastmilk; as well as other models such as mouse, rat, and porcine.

The platform routinely performs ELISA for the detection and quantification of targets in a sample. We integrate the use of robotic liquid handling for high throughput functionality with low sample variation.

To allow for the simultaneous detection of multiple targets in a single sample, the platform runs the Luminex xMAP® technology, a bead-based multiplexed ELISA assay system. The combination of advanced fluidics, optics and digital signal processing system with proprietary microsphere technology supports the multiplexed detection of more than 60 analytes and is best placed for the measurement of liquid samples such as human plasma or breast milk. With "Standard", "Exploratory" and "DropArray®" there are three different options for users to conduct their ideal assay depending on the required accuracy and available sample volume.

In addition to Luminex, the MAP platform performs biomarker detection on the Simoa HD-1 Analyzer (using the Quanterix single molecule array or Simoa® platform). This high-end ultra-sensitive technology allows for the detection of up to four analytes per well and is especially advantageous in measuring low-abundancy analytes in limited sample volumes.

Services

The following services are offered by the SIgN MAP platform:

Luminex Service ELISA Service Ultra Sensitivity Assay


Instrument Booking

Extracellular Flux Analyzer Seahorse XFe96 for metabolic profiling

The potential to study cellular bioenergetics in real-time provides crucial insights into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Agilent Seahorse XF technology uses label-free technology to measure changes in oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well format. Mitochondrial respiration and glycolysis are key functions that are investigated. Changes in cellular metabolism are measured kinetically and in real-time through the addition and mixing of compounds by the XF Analyzers.

The Extracellular Flux Analyzer Seahorse XFe96 machine at SIgN is available to external users (Non-academic users: $40/hr non-academic; Academic/ A*STAR users: $30/hr).

Mabtech IRIS™ reader

The MAP platform provides access to the Mabtech IRIS™ reader. The instrument is a next-generation, 4-colour FluroSpot and ELISpot reader that allows the analysis of traditional ELISpot assays as well as multiplexed FluoroSpot assays. For the latter, Mabtech offers various kits which allow, for instance, the simultaneous analysis of IL-5, IL-22, IL-17A and IFN-gamma (Click here for a complete list of Mabtech kits.). The proprietary RAWspot® technology extracts the volume of every spot, which corresponds to the relative amount of secreted analyte. Fixed camera settings result in objective RAW data, free of user bias. For self-operated access to Extracellular Flux Analyzer Seahorse XFe96 or the Mabtech IRIS™, please create a PPMS account to book the instrument.

For new users, kindly click here to create an account.

Existing users with accounts can login to the booking system here

Contact Us

MAP team at SIgN:
Wilson How & Norman Fernandez





For scientific questions, please contact:

Olaf RÖTZSCHKE
Olaf_Rotzschke@immunol.a-star.edu.sg
Head, Multiplex Analysis of Proteins (MAP)

For general enquiries/service engagements, please contact us at SIgN_IMP@immunol.a-star.edu.sg

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