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Electron Microscopy by A*STAR Microscopy Platform (AMP)

Exploring Ultrastructure and Beyond

The Electron Microscopy division of A*STAR Microscopy Platform (AMP|EM) provides tools and expertise in qualitative and quantitative ultrastructural analysis of biological and composite samples using transmission and scanning electron microscopy (TEM and SEM). We are founding members of SingaScope- a Singapore-wide microscopy infrastructure network. Services provided by the facility include training, consultation on sample preparation, sample processing, image acquisition and data analysis.

 

Scope of Services

  • Sample processing (RT and cryo)
  • Ultrathin (cryo-)sectioning
  • Ultrastructure analysis by (cryo-)TEM
  • Antigen detection by immuno-gold TEM
  • 3D surface ultrastructure analysis by SEM
  • 3D Electron tomography
  • Correlative light and electron microscopy
  • Stereology and quantitative image analysis
  • Training in instrument operation and sample processing for TEM/SEM

 

Sample Types

  • Nanoparticles and emulsions
  • Biomaterials and composites
  • Protein complexes
  • Viruses, bacteria and yeast cells
  • Purified cellular organelles
  • Membrane vesicles
  • Cells (suspensions or adherent)
  • Organoids, tissues and biopsies
  • Whole animals (embryos)

 

Key Equipment

Electron Microscopes:

  • High-resolution SEM (JEOL 6701F)
  • 120kV cryo-TEM (FEI Talos 120c)
  • 200kV cryo-TEM (JEOL 2200FS)

     

    Sample Processing Units

    • Plunge freezing EM GP2
    • High-Pressure Freezing HPM100
    • Freeze fracture and etching BAF060
    • Automated freeze-substitution AFS2 FSP
    • Critical Point Drying CPD030
    • Platinum/Gold/Carbon sputtering SCD050
    • (Cryo-)Ultramicrotomes UC7-FC7, UCT

     

    Recognition

    With a complete set of cryo-processing instrumentation from Leica Microsystems, our facility is recognised as the Leica Electron Microscopy Sample Preparation Centre of Excellence

     

    Analysis Type

    Qualitative and quantitative microscopy imaging of 2D or 3D ultrastructure, with or without antigen detection, down to nanometer spatial resolution.

     

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