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Light Microscopy by A*STAR Microscopy Platform (AMP)

Explore the Organisation and Dynamics of Your Samples

The Light Microscopy division of A*STAR Microscopy Platform (AMP|LM) provides expert staff and a wide range of light microscopy instrumentation and image analysis tools, facilitating research in the biomedical sciences. AMP is a founding member of SingaScope- a Singapore-wide microscopy infrastructure network.

 

Scope of Services

AMP|LM houses 20 high-end light microscopes and currently has over 250 regular users. AMP|LM also hosts the A*STAR Olympus Microscopy Suite where we have access to the latest technology and instrumentation from Olympus, as well as excellent support.

AMP|LM operates either by training users to be independent or as a service facility in which the microscopy is carried out with users by our experts. Once trained, users have access to our online booking system to plan their experiments.

 

Key Techniques

  • Brightfield microscopy
  • Widefield fluorescence
  • Slide scanning
  • Optical sectioning and 3D imaging
  • TIRF
  • Deconvolution
  • Confocal (galvo/resonant scanning and spinning disk)
  • Multiphoton
  • Lightsheet
  • Super resolution microscopy (3D-SIM, dSTORM)
  • F-techniques (FRAP, FRET, FLIM, FCS)
  • Live-cell imaging
  • High content screening and analysis
  • Image processing and analysis

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      Sample Types

      We have experience imaging a wide range of biological samples:

      • Bacteria
      • Yeast/fungi
      • Tissue sections
      • Cultured mammalian cells
      • Organotypic cultures
      • Whole-mount tissues
      • Intravital imaging of smalls animals

       

      Fluorophores

      Our fluorescence microscopes are equipped to image the full spectrum of fluorophores:

      • Blue (DAPI, Hoescht)
      • Cyan (CFP)
      • Green (FITC, Alexa488, GFP)
      • Yellow (YFP)
      • Red (TRITC, Alexa568, mCherry)
      • Far-red (Cy5, Alexa647)

      Our confocal microscopes are also capable of spectral imaging and unmixing.

         

        Microscopy scientific services are applicable for  Tech Access* grant subsidy. 
        *Terms and Conditions apply.

         

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