Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide. In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.
2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilizes RNA.
Highlights
• Two-color tracking of DNA, RNA migration during electrophoresis
• No DNA, RNA masking during gel exposure to UV light
• Denaturation of DNA, RNA due to the presence of formamide
Applications
• Preparation of DNA ladders, markers, and samples for loading on denaturing gels
• Preparation of RNA ladders, markers, and samples for loading on agarose or polyacrylamide gels