Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix is a preformulated, optimized universal 2X master mix for real-time PCR. The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 5 x 5 mL tube, sufficient for 2500 20-μL reactions (10 μL Master Mix per reaction). PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems.