Thermo Scientific B-PER Complete Bacterial Protein Extraction Reagent is an easy-to-use cell lysis reagent is a nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay). Depending on the particular application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.