BIORAD Bio-Safe Coomassie stain detects proteins in native, SDS-PAGE, and peptide gels without the use of methanol, acetic acid, or other hazardous agents. Bio-Safe Coomassie stain is a ready-to-use, single-reagent protein stain based on a modified Coomassie Blue G-250 stain that produces a linear response allowing quantitative analysis. Development is extremely easy. Simply soak the gel in the stain, and results begin to appear in 20 minutes. Sensitivity as high as 8 ng and greater band intensity are achieved by washing the gel in water after staining.