Each kit contains:
• Caspase-Glo® 8 Substrate (G815A, 1 x 1 bottle)
• Caspase-Glo® 8 Buffer (G817A, 1 x 10 ml)
• MG-132 Inhibitor (G932B, 1 x 30 ul)
A Luminescent Assay That Measures Caspase-8 Activity. The assay provides a proluminogenic caspase-8 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 8 Reagent in an "add-mix-read" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable "glow-type" luminescent signal and improves performance across a wide range of assay conditions.