DMB Sialic Acid Labeling Kit (formerly ProZyme)
Release and derivatization of sialic acids with 1,2-diamino4,5-methylenedioxybenzene (DMB) for separation by liquid chromatography.
Comparison may be made against the included sialic acid reference panel (SARP, p/n GKRP-2503), which includes:
Neu5Gc (NGNA)
Neu5Ac (NANA)
Neu5,7Ac2
Neu5Gc,9Ac
Neu5,9Ac2
Neu5,7(8)9Ac3.
Up to 20 samples may be processed (2 batches of 10).
Count : 10
Protein Load Range : 1-10 µg
Equipment and Reagents Provided by User :
Heating block or oven (set at 50EC for labeling, 80EC for release reaction)
HPLC system for analysis
GlykoSep R HPLC column
Water, HPLC grade
Methanol, HPLC grade
Acetonitrile, HPLC grade