DNeasy Blood & Tissue Kit (250) contains 250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
DNeasy Blood & Tissue Kit for purification of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses
- Standardized method for a variety of sample types
- High yields even from specialized samples
- High-quality DNA
- Optimized protocols for a range of starting materials
- Spin-column and 96-well high-throughput formats
DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Purification of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube.
DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.
The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.
Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.
Applications
DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:
- Life science research
- Livestock breeding
- Pedigree genotyping
- Veterinary pathogen research
- Routine applied testing