Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG; it has been affinity-purified and conjugated to horseradish peroxidase. When used in a Western blot at a 1:10,000 dilution with colorimetric detection, Donkey Anti-Goat IgG, HRP Conjugate shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG and extracts of PC12 cells. It detects 100ng of goat IgG with minimal background signal.