Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR setup.
The master mix retains all features of DreamTaq DNA Polymerase and is capable of robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
DreamTaq Green PCR Master Mix (2X) additionally includes 2X DreamTaq Green Buffer with a density reagent and two dyes for monitoring electrophoresis progress.
Features
Robust amplification with minimal optimization
High yields of PCR products
Higher sensitivity compared to conventional Taq DNA polymerase
Amplification of targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
Incorporates modified nucleotides, but does not incorporate dUTP
Direct loading of PCR product on gel with green format
Applications
Routine PCR amplification of DNA fragments up to 6 kb
High throughput PCR
Genotyping