Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Pierce ECL Plus Western Blotting Substrate characteristics include:
• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)
Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.
Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.