Effectene Transfection Reagent (4 x 1 ml) contains 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
Effectene Transfection Reagent for DNA transfection of primary cells and sensitive cell lines
- High efficiency in the presence of serum
- Efficient transfection with low DNA amounts
- Far lower cytotoxicity and gentler than many alternatives
- Suitable for high-throughput screening
Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines.
Higher transfection efficiencies of plasmid DNA are achieved with Effectene Transfection Reagent than with other reagents when used with the recommended procedures. Effectene Transfection Reagent is suitable for transfecting sensitive cell lines with oligonucleotides and is particularly effective for primary cells. Many cell lines and primary cells have been successfully transfected using Effectene Reagent; cell type-specific transfection protocols are available. Cytotoxicity is minimal because transfection with Effectene Reagent can be performed in the presence of serum and requires low amounts of DNA.
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Transfection Reagent is available in bulk quantities.
Applications
Effectene Transfection Reagent is suitable for transient and stable transfection of a broad range of cell types.