The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001).
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
Features of the ExpiFectamine CHO Transfection Kit include:
Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discovery
Achieve >90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
Uses one-third to one-half less plasmid DNA than other transfection reagents
Scale growth and transfection in culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately.