Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 μg/mL. This product is supplied as a 50 mg/mL solution.
Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Hygromycin B has a different mode of action than Geneticin™, Blasticidin S, or Zeocin™ selection reagents, making it an excellent choice for dual-selection experiments