Hank's Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.
This HBSS is modified as follows:
With Calcium, Magnesium and Glucose
Without Phenol Red and Sodium Pyruvate