HiSpeed Plasmid Maxi Kit for 25 HiSpeed Maxi Tips, 25 QIAfilter Maxi Cartridges, 25 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
HiSpeed Plasmid Maxi Kit for ultrafast purification of up to 750 µg transfection-grade plasmid or cosmid DNA
- Less than 60 minutes prep time
- Lysate clearing and isopropanol precipitation without centrifugation
- No risk of DNA pellet loss during precipitation
- Up to 750 µg yield of high-copy plasmid DNA
- LyseBlue for optimum lysis and maximum DNA yield
HiSpeed Plasmid Maxi Kits provide QIAfilter Cartridges, HiSpeed Tips, and QIAprecipitator modules for fast large-scale plasmid preparation. Syringe-format QIAfilter and QIAprecipitator modules replace centrifugation steps in the classic anion-exchange procedure, making plasmid purification faster and more convenient. Up to 750 µg high-copy plasmid DNA can be purified from 150–250 ml culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.) HiSpeed Tip design allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster.
The unique anion-exchange resin in HiSpeed Tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation.
Plasmid DNA purified with HiSpeed Plasmid Maxi Kits provides excellent results in all applications, such as:
- Transfection
- Cloning
- PCR
- Sequencing
- Plasmid-mediated gene silencing