HiSpeed Plasmid Midi Kit for 25 HiSpeed Midi Tips, 25 QIAfilter Midi Cartridges, 25 QIAprecipitator Midi Modules plus Syringes, Reagents, Buffers
HiSpeed Plasmid Midi Kit ultrafast purification of up to 200 µg transfection-grade plasmid or cosmid DNA
- Less than 45 minutes prep time
- Lysate clearing and isopropanol precipitation without centrifugation
- No risk of DNA pellet loss during precipitation
- Up to 200 µg yield of high-copy plasmid DNA
- LyseBlue for optimum lysis and maximum DNA yield
HiSpeed Plasmid Midi Kits provide QIAfilter Cartridges, HiSpeed Tips, and QIAprecipitator modules for fast large-scale plasmid preparation. Syringe-format QIAfilter and QIAprecipitator modules replace centrifugation steps in the classic anion-exchange procedure, making plasmid purification faster and more convenient. Up to 200 µg high-copy plasmid DNA is purified from 50–150 ml culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.) HiSpeed Tip design allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster.
The unique anion-exchange resin in HiSpeed Tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation.
Plasmid DNA purified with HiSpeed Plasmid Midi Kits provides excellent results in all applications, such as:
- Transfection
- Cloning
- PCR
- Sequencing
- Plasmid-mediated gene silencing
For more product information please visit:
http://www.qiagen.com/Products/Catalog/Sample-Technologies/DNA-Sample-Technologies/Plasmid-DNA/HiSpeed-Plasmid-Midi-Kit#productdetails