HotStarTaq DNA Polymerase (1000 U) for 4 x 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
HotStarTaq DNA Polymerase for highly specific amplification with minimal optimization
- Minimal optimization requirements
- High PCR specificity
- Easy handling and room-temperature setup
HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. HotStarTaq DNA Polymerase is supplied with the innovative dual-cation QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided.
Applications
HotStarTaq DNA Polymerase is suitable for a wide variety of applications, including challenging applications, such as amplification of:
- Complex genomic templates
- Complex cDNA templates (e.g., RT-PCR)
- Very low-copy targets (e.g., single-cell PCR)
- Reactions with multiple primer pairs