Rapid protocol: 30 min/6 preps; High purity RNA (A260/A280 ratio 1.9 - 2.1); RNA integrity number (RIN) >9; Extra filters (shredders) included to enhance sample homogenization; DNase I included for complete removal of genomic DNA; Isolation of RNA from:Animal tissue, Eukaryotic cells, Bacterial cells, Yeast, Biological fluids (cell-free); Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR.