Description:
KOD FX Neo is based on the DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD FX Neo provides greater efficiency and elongation capabilities than conventional PCR enzymes. In particular, KOD FX Neo shows greater amplification success from crude specimens.
KOD FX Neo is the improved version of the previous KOD FX (Code No. KFX-101). This product contains a unique "elongation enhancer" that suppresses the "plateau effect", enabling greater elongation rates and capabilities.
The KOD FX Neo enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR. KOD FX Neo generates blunt-end PCR products because of its 3'→5' exonuclease (proof-reading) activity.
Features:
This enzyme is effective for amplification from crude samples (e.g. biological samples, foodstuffs, soil extract, etc). Various samples or lysates can be used directly as templates.
"Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 40 kb from human genomic DNA) compared to conventional PCR. This enzyme is useful for amplifying difficult targets, such as high G/C, A/T, and/or long targets.
Various microorganisms (e.g. yeast, fungus, gram-positive bacteria) can be directly used as templates for PCR.
The PCR error ratio of KOD FX Neo is about 10 times less than that of Taq DNA polymerase and is equal to the previous version (KOD FX).
*Mutation frequency was measured by sequence analysis of human β-globin gene
　products amplified from human genomic DNA via TA cloning.
Applications:
Genotyping (Amplification from crude sample)
Direct amplification from;
-Whole blood, cultured cell, mouse tail, mouse toe , plant and food stuff etc.
-Various kinds of lysate from mouse tail, plant, fish fin, soil etc.
- Gram-positive bacteria, fungus, yeast etc.
Efficient amplification of difficult targets (high G/C, long ) with fast mode.
High fidelity PCR
Components:
This reagent includes the following components for 200 reactions, 50 µl total reaction volume:
KOD FX Neo (1.0 U / µl) * 200 µl × 1
2 x PCR Buffer for KOD FX Neo** 1.7 ml × 3
2 mM dNTPs 1 ml × 2
*The enzyme solution contains anti-KOD DNA polymerase antibodies that neutralize the polymerase and 3'→5' exonuclease activities.
** 2x PCR Buffer for KOD FX Neo is a liquid (not frozen) when stored at -20ºC. Although it does freeze below -20ºC, the quality is not affected.
Protocol: http://www.toyobo-global.com/seihin/xr/lifescience/support/manual/KFX-201.pdf