Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. It can co-deliver DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes. Lipofectamine Stem reagent is compatible with a variety of media systems, including feeder-free, helping support and simplify your stem cell culture workflow. Researchers can achieve up to 80% or better transfection efficiency in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in mesenchymal stem cells (MSCs).
Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation.