miRNeasy Micro Kit (50) contains 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers
- Effective microRNA (miRNA) and total RNA purification, even from small samples
- Efficient enrichment of miRNA and RNAs <200 nucleotides
- Protocols for copurification or isolation of separate fractions
- High-purity RNA suitable for all downstream applications
- Automatable protocols and high-throughput processing in 96-well format
miRNeasy Kits combine phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
miRNeasy Micro and Mini Kits are automatable on the QIAcube Connect. The miRNeasy 96 Kit enables vacuum and/or spin processing.
Application
- RNA-seq
- Quantitative, real-time RT-PCR (e.g., using the miScript PCR System)
- Northern blot analysis
- Microarray analysis