The MycoAlertTM PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlertTM PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlertTM PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlertTM PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
The MycoAlertTM PLUS Mycoplasma Detection Kit is the next generation MycoAlertTM Kit which provides higher light output and this allows for
use with less sensitive plate luminometers or multifunctional readers
testing of unused media, media supplements or water
The MycoAlertTM PLUS Mycoplasma Detection Kit is suited for tube luminometers, plate luminometers and multifunctional readers.