N-2 Supplement is a chemically-defined, serum-free supplement based on Bottenstein’s N-1 formulation. It is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system. N-2 Supplement is provided as a 100X concentrate and is intended for use with Neurobasal Medium supplemented with growth factors such as bFGF and EGF. It may also be used with DMEM.