Each kit contains:
• Pfu DNA Polymerase (M774A, 1 x 100 u)
• Pfu 10X Reaction Buffer w/20mM MgSO4 (M776A, 1 x 1.2 ml)
Conc. 2-3u/ul. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´?3´ direction in presence of Mg. It also possesses 3´?5´ exonuclease (proofreading) activity; recommended for high-fidelity synthesis. Produces blunt-ended fragments.