Each kit contains:
• pGEM®-T Easy Vector (A137A, 1 x 1.2 ug, 50 ng/ul)
• Control Insert DNA (A363A, 1 x 12 ul)
• 2X Rapid Ligation Buffer (C671A, 1 x 200 ul)
• T4 DNA Ligase (M180A, 1 x 100 u)
For direct cloning of PCR products into a convenient plasmid vector (TA Cloning). The systems include pGEM®-T Vector Control DNA, T4 DNA Ligase, 2X Rapid Ligation Buffer and pGEM®-T Easy Vector. These vectors have been linearized and have had 3?-terminal thymidine nucleotides added. The pGEM®-T Easy Vector Systems offer added convenience of recognition sites for EcoRI and NotI flanking the insertion site.