Invitrogen™ Phasemaker™ Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of a TRIzol® Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA.
• Pre-dispensed, 2 mL tubes
• Exclusively for RNA extraction
• For use with TRIzol Reagent, TRIzol® LS Reagent, and TRIzol® Plus Purification Kit only
• Simplifies removal of aqueous phase
• Helps increase RNA yield up to 30%
• Does not interfere with downstream experiments
The polymer in Phasemaker Tubes is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation it positions itself between these two layers. As a thick polymer, it creates a tight seal between the two phases, easing removal of the aqueous phase and helping to reduce the risk of carrying over contaminants from the organic layer, thereby increasing RNA recovery up to 30%.