The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard.
Features of the BCA Protein Assay Kit include:
Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
Sensitive—detect down to 5 μg/mL with enhanced protocol
No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve