GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus , binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.
Note: High temperature heating is not recommended. The agarose melts above 65°C.
Store at 2-8°C, do NOT freeze.
Key Features:
High IgG-binding capacity
Resin is reusable up to 10 times with no significant loss of binding capacity.
https://www.genscript.com/kit/L00210_10-Protein_A_Resin.html