The PureLink™ HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without the use of any organic solvents or cesium chloride, and contains low endotoxin levels.The kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials.
PureLink™ HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1-3 mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1-1 EU/ μg, when cloning high copy number plasmids. Typically, DNA isolated using the PureLink™ HiPure Plasmid Miniprep Kit has an A260/A280 > 1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.