QIAFilter Plasmid Midi Kit for 25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges
QIAFilter Plasmid Midi Kit for fast purification of up to 100 µg transfection-grade plasmid or cosmid DNA
- Lysate clearing without centrifugation
- Reduced plasmid purification time
- High yields of plasmid DNA
- LyseBlue reagent for optimum lysis and maximum DNA yield
The QIAfilter Plasmid Midi Kit combines QIAfilter Midi cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tip 100 anion-exchange tips for efficient plasmid purification. Using the syringe-format QIAfilter Midi Cartridge and the QIAGEN-tip 100, up to 100 µg transfection-grade plasmid DNA can be purified from 25–50 ml culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.)
Plasmid DNA purified with QIAfilter Plasmid Midi Kits provides excellent results in all applications, such as:
- Transfection
- Cloning
- PCR
- In vitro transcription