QIAGEN Plasmid Plus Midi Kit (25) contains 25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges
This kit is based on vacuum protocol, hence, it HAS to be used together with QIAvac 24 Plus* Vacuum Manifold (#19413) and a vacuum pump. The QIAvac Connecting System** (#19419) is optional.
QIAGEN Plasmid Plus Midi Kit for the fastest, most convenient purification of up to 250 μg transfection-grade plasmid DNA
- Rapid purification of up to 24 samples in 20 minutes
- Easy vacuum-based parallel processing
- Low elution volumes for highly concentrated DNA
- Very low endotoxin levels due to special wash buffer
- Suitable for all applications
The QIAGEN Plasmid Plus Midi Kit enables ultrafast, large-scale purification of up to 250 μg of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing the need for hands-on time. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The QIAGEN Plasmid Plus Midi Kit also features a novel wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.
The QIAGEN Plasmid Plus Midi Kit provides transfection-grade plasmid DNA highly suited for all applications, such as:
- Transfection into most cell lines (e.g, sensitive cell lines such as Huh-7)
- Restriction digestion
- Cloning
- In vitro transcription and translation
- Sequencing