QIAprep Spin Miniprep Kit (250) contains 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)

- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
- Even higher yields with the High-Yield Supplementary Protocol
- Improved QIAprep 2.0 Spin Column
- GelPilot loading dye for convenient sample analysis
The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA for various molecular biology applications. QIAprep 2.0 Spin Columns contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep 2.0 Spin Columns is immediately ready to use – there is no need to precipitate, concentrate, or desalt. To enable faster and more convenient sample processing and analysis, gel loading dye is provided in the kit. GelPilot Loading Dye contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The kit is automatable on the QIAcube Connect. For optimal results, we recommend pairing this kit with the QIAvac 24 Plus system.
Applications
- PCR
- Restriction digestion
- Ligation and transformation
- Sequencing
- Screening