QIAquick Gel Extraction Kit (250) for gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
QIAquick Gel Extraction Kit for gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions
- Up to 95% recovery of ready-to-use DNA
- Fast and convenient procedure
- Cleanup of DNA up to 10 kb in three easy steps
- Gel loading dye for convenient sample analysis
The QIAquick Gel Extraction Kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of DNA (see figure High recoveries from gels). Using a microcentrifuge or vacuum manifold, DNA ranging from 70 bp to 10 kb is purified from 1–24 samples. Purified DNA can be used, for example, in sequencing. DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System. The procedure can be fully automated on the QIAcube.
Applications
DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.