The Qubit dsDNA BR (Broad-Range) Assay Kit, designed for use with Qubit 4 or Qubit Flex Fluorometers, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 4,000 ng/μL, providing a detection range of 4-4,000 ng.
The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometers. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. To perform the assay, simply dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a Qubit Fluorometer.