The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 5 pg/μL to 120 ng/μL, providing a detection range of 0.1−120 ng.
The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometers. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. To perform the assay, simply dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a Qubit Fluorometer.