Each kit contains:
• Annexin V-SmBiT Reagent (1,000X) (J700A, 1 x 25 ul)
• CaCl2 (1,000X) (J750A, 1 x 250 ul)
• Annexin V-LgBiT Reagent (1,000X) (J800A, 1 x 25 ul)
• Annexin V NanoBiT® Substrate, 1,000X (JA100A, 1 x 25 ul)
• Necrosis Detection Reagent, 1,000X (JA101A, 1 x 25 ul)
The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay measures the real-time exposure of phosphatidylserine (PS) on the outer leaflet of cell membranes during the apoptotic process. Annexin V luciferase fusion proteins supplied in the assay reagent bind to PS during early apoptosis and are detected with a simple luminescence signal. The assay reagent also includes a DNA-binding dye, which enters the cell and generates a fluorescent signal upon loss of membrane integrity. The combination and timing of luminescent (annexin V binding) and fluorescent (DNA release) signals is used to differentiate secondary necrosis occurring during late apoptosis from necrosis caused by other cytotoxic events.