High efficient cDNA synthesis master mix for real-time PCR with gDNA remover
Features:
"Genomic DNA degradation step" and "cDNA synthesis step" can be achieved sequentially in approximately 30 min.
The master mix reagent contains random and oligo dT primers optimized for efficient reverse transcription.
Control, no reverse transcription experiments (no RT-control) can be performed with 5x RT Master Mix II no-RT control.
Application:
cDNA synthesis for real-time PCR
Componenrts:
gDNA Remover 10 µl
4x DN Master Mix 440 µl
5x RT Master Mix II 400 µl
5x RT Master Mix II no RT-Control 40 µl
Nuclease-free water 1000 µl × 2
Protocol: http://www.toyobo-global.com/seihin/xr/lifescience/support/manual/FSQ-301.pdf