Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications
• Removal of mRNA prior to synthesis of second strand cDNA
• RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
• Site-specific cleavage of RNA
• Studies of in vitro polyadenylation reaction products