Gibco™ StemFlex™ Medium supports the robust expansion of feeder-free pluripotent stem cells (PSCs) and is optimized to support novel applications, including single-cell passaging, gene editing, and reprogramming. StemFlex Medium’s unique formulation offers the convenience of a flexible feeding schedule (including weekend-free options) and also the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.