SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III RT in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of that enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity. SuperScript IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently.
SuperScript IV RT offers advantages at standard as well as elevated temperatures over cloned AMV and ThermoScript RT, including higher sensitivity, inhibitor tolerance, and ability to work with degraded RNA.