Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) HRP substrate with stable light output for mid-femtogram-level detection with CCD-based imagers.
SuperSignal West Dura Extended Duration Substrate characteristics include:
• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)