SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR Green I stain is ideal for use with laser scanners.
SYBR Green I stain provides:
• Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels
• Ease of use—gels soaked in diluted stain can be visualized without desalting
• Compatibility—with UV transilluminators, gel documentation systems, and laser scanners
• Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection