Each kit contains:
• T4 DNA Ligase 10X Buffer (C126A, 2 x 500 ul)
• T4 DNA Ligase (M180B, 1 x 500 u)
Contains 500 Weiss units, at 1–3u/?l. One Weiss unit=67 Cohesive End Ligation Units. T4 DNA Ligase catalyzes the joining of 2 DNA strands between the 5´-P and the 3´-OH groups of adjacent nucleotides in either cohesive-ended or blunt-ended configuration. The enzyme has been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.