Each kit contains:
• T4 RNA Ligase (M105A, 1 x 500 u)
• T4 RNA Ligase 10X Buffer (M107A, 1 x 500 ul)
Conc. 10u/ul .T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient). T4 RNA Ligase also catalyzes the addition of [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA.